PE anti-human CD255 (TWEAK) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1608	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

hTWEAK/2PK-3 transfectant cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Flow Cytometry: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 308304) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
ELISA Detection: The biotinylated CARL-1 antibody is useful as the detection antibody in sandwich ELISA for measuring human TWEAK protein, in combination with CARL-2 antibody (Cat. No. 308402) used as ELISA capture.
Neutralization^1,2: In a dose-dependent manner, CARL-1 is more effective than CARL-2¹. The LEAF? purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 308307).

Application References (PubMed link indicates BioLegend citation)

Nakayama, M., et al. 2000. J. Exp. Med. 192:1373. (Neut)
Nakayama, M., et al. 2003. J. Immunol. 170:341. (Neut)
Zou H, et al. 2011. Gynecol Oncol.123:123. PubMed.

Product Citations

Kenty JH, et al. 2021. STAR Protocols. 2(3):100675. PubMed

RRID

AB_314709 (BioLegend Cat. No. 308305)

Antigen Details

Structure: TNF superfamily; 17 kD (Mammalian)
Distribution: Normal tissue and tumor cells at low levels
Function: Promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, apoptosis
Bioactivity: Weak apoptotic induction; mediate CD4⁺ T-cell killing of macrophages; induces IL-6, IL-8, ICAM-1 in astrocytes; HUVEC proliferation
Cell Sources: Transcripts widely expressed and abundant in most tissues; protein efficiently secreted
Cell Targets: Macrophages, astrocytes, endothelial cells
Receptors: Apo-3 (DR3, WSL-1, TRAMP, LARD); TWEAKR
Biology Area: Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Chicheportiche, Y., et al. 1997. J. Biol. Chem. 272:32401.
3. Kaplan, M., et al. 2000. J. Immunol. 164:2897.
4. Saas, P., et al. 2000. Glia 32:102.
5. Wiley, S., et al. 2001. Immunity 15:837.
Regulation: Type II integral membrane protein readily cleaved from cell surface
Gene ID: 8742 View all products for this Gene ID
UniProt: View information about CD255 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们