Ultra-LEAF™ Purified anti-mouse CD223 (LAG-3
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD223 (LAG-3
- 产品类别：
- 抗体
- 产品编号：
- 125231
- 产品应用：
- 125231
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Murine CD223-Ig fusion protein
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
FA, IP, ELISA - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
- Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
- Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
- RRID
- AB_2813999 (BioLegend Cat. No. 125231) AB_2814000 (BioLegend Cat. No. 125232) AB_2814001 (BioLegend Cat. No. 125233) AB_2814002 (BioLegend Cat. No. 125234) AB_2814003 (BioLegend Cat. No. 125235) AB_2814004 (BioLegend Cat. No. 125236)
