GoInVivo™ Purified anti-mouse CD223 (LAG-3)
- 产品名称:
- GoInVivo™ Purified anti-mouse CD223 (LAG-3)
- 产品类别:
- 抗体
- 产品编号:
- 125216
- 产品应用:
- 125216
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Murine CD223-Ig fusion protein
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <1.0 EU/mg of the protein. (<0.01 pg/?g of the protein) as determined by the LAL test.
- Preparation
- The GoInVivo? antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
FA, IP, ELISA - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.
- Application Notes
GoInVivo? products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo? Webpage.
- Application References
(PubMed link indicates BioLegend citation) -
- Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
- Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
- Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
- RRID
- AB_2566284 (BioLegend Cat. No. 125216) AB_2566285 (BioLegend Cat. No. 125217) AB_2566286 (BioLegend Cat. No. 125218) AB_2566095 (BioLegend Cat. No. 125213) AB_2566096 (BioLegend Cat. No. 125214) AB_2566097 (BioLegend Cat. No. 125215)