Alexa Fluor® 647 anti-human CD8a Antibody
- 产品名称：
- Alexa Fluor® 647 anti-human CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 372906
- 产品应用：
- 372906
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- A 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of the alpha chain of the human CD8 molecule.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
ICFC, 3D IHC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 5.0 - 10 ?g per million cells in 100 ?l volume.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include activation3, flow cytometry4, immunohistochemical staining of frozen tissue sections2, and Western blotting5.
- Application References
(PubMed link indicates BioLegend citation) -
- McQuitty E, et al. 2014. J. Cutan. Pathol. 41:88. (IHC-P)
- Petukhova L, et al. 2010. Nature 466:113. (IHC-F)
- Clement M, et al. 2011. J. Immunol. 187:654. (Activ)
- MarTchal R, et al. 2010. BMC Cancer 10:340. (FC)
- Pontiggia L, et al. 2008. J. Invest. Dermatol. 129:480. (WB)
- Product Citations
-
- Pelka K, et al. 2021. Cell. 184(18):4734-4752.e20. PubMed
- Chen B, et al. 2021. Cell. 184:6262. PubMed
- RRID
- AB_2650712 (BioLegend Cat. No. 372906)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β.
- Distribution
-
Majority of thymocytes, T cell subset, NK cells.
- Function
- MHC class I co-receptor, thymic differentiation, T-cell activation.
- Ligand/Receptor
- MHC class I molecules.
- Cell Type
- NK cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1.?McQuitty E,?et al.?2014.?J. Cutan. Pathol.?41:88.
- Gene ID
- 100387674 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
