Ultra-LEAF™ Purified anti-human IL-12/IL-23 -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2184	1

Product Details

Verified Reactivity

Human, IL-12/IL-23 p40 subunit (monomer, homodimer and heterodimer IL-12 p35/p40 or IL-23 p19/p40)

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

COS-expressed p40 subunit of human IL-12

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

ELISA-C - Quality tested
ICFC, IP, Neut, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 1.0 - 8.0 ?g/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Neutralization^1,2,3: The LEAF?Purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-12 bioactivity (Cat. No. 508804).

Application References (PubMed link indicates BioLegend citation)

D'Andrea A, et al. 1992. J. Exp. Med. 176:1387.
Goodier M, et al. 2000. J. Immunol. 165:139.
Aragane Y, et al. 1994. J. Immunol. 153:5366.

RRID

AB_2810643 (BioLegend Cat. No. 508807) AB_2810644 (BioLegend Cat. No. 508808)

Antigen Details

Structure: Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity: IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of T_H1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me
Cell Sources: Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets: T cells, NK cells, PBL
Receptors: IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Oppmann B, et al. 2000 Immunity. 13:715.
6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002 J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002 J. Immunol. 168:5448.
9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49.
Regulation: Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Gene ID: 3593 View all products for this Gene ID
UniProt: View information about IL-12 p40 on UniProt.org

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