Biotin anti-human IL-12/IL-23 p40 (monomer, dimer,-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1212	1

Product Details

Verified Reactivity

Human, IL-12/IL-23 p40 subunit (monomer, homodimer and heterodimer IL-12 p35/p40 or IL-23 p19/p40)

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

COS-expressed p40 subunit of human IL-12

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5 - 2.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12 p40 recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. To obtain a linear standard curve, serial dilutions of IL-12 p70 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 ?g/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Neutralization^1,2,3: The LEAF?Purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-12 bioactivity (Cat. No. 508804).

Additional Product Notes

ELISA or ELISPOT Detection¹: The biotinylated C8.6 antibody is useful as a detection antibody for an IL-12 p40 specific sandwich ELISA or ELISPOT assay, when used in conjunction with the C8.3 antibody (Cat. No. 501702/501704) as the capture antibody. The biotinylated C8.6 antibody is useful as a detection antibody for an IL-12 p70 specific sandwich ELISA or ELISPOT assay, when used in conjunction with the Purified 7B12 antibody (Cat. No. 511002/511004) as the capture antibody. Each lot of this antibody is quality control tested by ELISA assay.

Application References (PubMed link indicates BioLegend citation)

D'Andrea A, et al. 1992. J. Exp. Med. 176:1387.
Goodier M, et al. 2000. J. Immunol. 165:139.
Aragane Y, et al. 1994. J. Immunol. 153:5366.

Product Citations

Haug V, et al. 2020. Transplantation. e260:104. PubMed
Amici SA, et al. 2018. Front Immunol. 16:e2005090. PubMed

RRID

AB_315528 (BioLegend Cat. No. 508801) AB_315529 (BioLegend Cat. No. 508802)

Antigen Details

Structure: Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity: IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of T_H1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me
Cell Sources: Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets: T cells, NK cells, PBL
Receptors: IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Oppmann B, et al. 2000 Immunity. 13:715.
6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002 J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002 J. Immunol. 168:5448.
9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49.
Regulation: Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Gene ID: 3593 View all products for this Gene ID
UniProt: View information about IL-12 p40 on UniProt.org

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