Purified anti-human IL-12/IL-23 p40 (monomer, homo-上海雅吉生物科技有限公司

规格	价格	库存
500µg	￥ 2748	1

Product Details

Verified Reactivity

Human, IL-12/IL-23 p40 - specific (not reactive with IL-12 p70 and IL23 p19/p40)

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

COS-expressed p40 subunit of human IL-12

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2 ?g/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. To obtain a linear standard curve, serial dilutions of IL-12 (p40) recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate.

Application Notes

ELISA or ELISPOT Capture: The purified C8.3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated C8.6 antibody (Cat. No. 508802/508805) as the detecting antibody. The LEAF? purified antibody is suggested for ELISPOT capture. This assay detects specifically human IL-12/IL-23 p40 protein, without interference by IL-12 p70 or IL-23 p19 proteins.
Note: For testing human IL-12/IL-23 p40 (monomer, dimer) in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 430701 to 430706) are specially developed and recommended. For testing human IL-12 p70 in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 431701 to 431706) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

D'Andrea A, et al. 1992. J. Exp. Med. 176:1387.
D'Andrea A, et al. 1993. J. Exp. Med. 178:1041.

Product Citations

Stensland ZC, et al. 2022. iScience. 25:103626. PubMed
Amici SA, et al. 2018. Front Immunol. 16:e2005090. PubMed

RRID

AB_2255186 (BioLegend Cat. No. 501702)

Antigen Details

Structure: Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity: IL-12 p70 (p40:p35) induces IFN-γ, TNF-α production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of T_H1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by hu
Cell Sources: Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets: T cells, NK cells, PBL, mature dendritic cells
Receptors: IL-12Rβ1 binds p40 from both IL-12 and IL-23; dimeric with IL-12Rβ2 binds p35; IL-23 specific receptor subunit, IL-23R binds p19
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992. Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993. Immunol. Today. 14:335.
5. Oppmann B, et al. 2000. Immunity. 13:715.
6. Aggarwal S, et al. 2003. J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002. J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002. J. Immunol. 168:5448.
9. Lankford CS, et al. 2003. J. Leukoc. Biol. 73:49.
Regulation: Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function
Gene ID: 3593 View all products for this Gene ID
UniProt: View information about IL-12 p40 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们