Ultra-LEAF™ Purified anti-mouse CD314 (NKG2D-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2316	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Soluble mouse NKG2D

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for the relevant formats) applications include: blocking^1,2,4,5 and inducing redirected cell lysis^1,2. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115704) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 115713 & 115714).

Application References (PubMed link indicates BioLegend citation)

Gilfillan S, et al. 2002. Nat. Immunol. 3:1150. (Block)
Ho EL, et al. 2002. J. Immunol. 169:3667. (Block)
Maeda M, et al. 2004. J. Immunol. 172:6115. (FC)
Andoniou CE, et al. 2005. Nature Immunology 6:1011. (Block)
O'Leary JG, et al. 2006. Nature Immunology 7:507. (Block)
Tang X, et al. 2006. J. Immunol. 177:7645. (FC)

RRID

AB_2832393 (BioLegend Cat. No. 115713) AB_2832394 (BioLegend Cat. No. 115714)

Antigen Details

Structure: C-type lectin
Distribution: NK and NK-T cells
Function: NK cytolytic killing of target cells expressing NKG2D ligands, costimulation NK cells
Ligand/Receptor: Minor histocompatibility molecule H60, retinoic acid early inducible 1 (RAE-1) family
Cell Type: NK cells, NKT cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
2. Vance RE, et al. 1998. J. Exp. Med. 188:1841.
3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755.
4. Yokoyama WM. 1998. Curr. Opin. Immunol. 10:298.
5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150.
6. Ho EL, et al. 2002. J. Immunol. 169:3667.
Gene ID: 27007 View all products for this Gene ID
UniProt: View information about CD314 on UniProt.org

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