上海细胞库
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
| 规格 | 价格 | 库存 |
|---|---|---|
| 1mL | ￥ 7248 | 1 |
IHC-P, ICC, IP
FC, WB - Reported in the literature, not verified in house
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
? IHC: ≥1:20 for Biotin based detection systems such as USA Ultrastreptavidin Detection (Cat. No. 929501)
Tissue Sections: Formalin-fixed, paraffin-embedded tissues.
Pretreatment: Not Required.
Incuabation: 20-60 minutes at room temperature.
?
This antibody is effective in immunoblotting, immunohistochemistry (IHC), immunocytochemistry (ICC) and immunopurification (IP).
Positive Cell Lines: Drug-sensitive parental cell lines and their multidrug-resistant derivatives.
Positive Tissues: Human liver (positive staining detected along luminal surfaces of bile canaliculi) or human colon (positive staining localized to luminal surface of secretaory epithelium).
C494 detects a gene-specific, internal cellular epitope (PNTLEGN) present only on the Mdr1 isoform of P-glycoprotein. C494 does not cross react with Mdr3. C494 has been shown to cross-react with Pyruvate Carboxylase (PC), an abundant 130kDa mitochondrial enzyme, on both immunoblots and immunohistochemical tissue sections. Unequivocal plasma membrane patterns of immunostaining represent true P-glycoprotein expression. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P-glycoprotein.
