Purified anti-human/mouse/rat CD278 (ICOS) Antibod
- 产品名称：
- Purified anti-human/mouse/rat CD278 (ICOS) Antibod
- 产品类别：
- 抗体
- 产品编号：
- 313502
- 产品应用：
- 313502
- Verified Reactivity
- Human, Mouse, Rat
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus, Pig
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse T cell clone D10.G4.1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IP, Costim - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that reagents be titrated for optimal performance in the particular application.
- Application Notes
- The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1,?in vitro?costimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 313512).
- Application References
(PubMed link indicates BioLegend citation) -
- Redoglia V,?et al.?1996.?Eur. J. Immunol.?26:2781. (FC IP Costim)
- Yagi J,?et al.?2003.?J. Immunol.?171:783. (FC)
- Arimura Y,?et al.?2002.?Int. Immunol.?14:555. (Costim)
- Arimura Y,?et al.?2004.?J. Biol. Chem.?279:11408. (Costim)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
- Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
- Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
- Sullivan KD, et al. 2021. Cell Reports. 36(7):109527. PubMed
- Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
- Son YM, et al. 2020. Eur J Immunol. 50:1067. PubMed
- Mann ER, et al. 2020. Sci Immunol. :5. PubMed
- Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
- Kim SJ, et al. 2017. Nat Immunol. 18:1016. PubMed
- Kinosada H, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006120. PubMed
- Galbraith MD, et al. 2021. eLife. 10:00. PubMed
- Sprouse ML, et al. 2018. JCI Insight. 3:e97322. PubMed
- Tripathi T, et al. 2019. Immunohorizons. 0.201388889. PubMed
- Sun Y, et al. 2020. J Immunol. 205:3358. PubMed
- Brenchley J, et al. 2012. Blood. 120:4172. PubMed
- Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
- Gubin MM, et al. 2018. Cell. 175:1014. PubMed
- Macmillan ML, et al. 2021. Blood Adv. 5:1425. PubMed
- Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
- Levin SD, et al. 2020. Front Immunol. 10:3086. PubMed
- Schwabenland M, et al. 2021. Immunity. . PubMed
- Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
- Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
- Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
- Stensland ZC, et al. 2022. iScience. 25:103626. PubMed
- Kaw S, et al. 2020. EMBO J. 39:e105594. PubMed
- Prabakaran T, et al. 2021. EBioMedicine. 66:103314. PubMed
- RRID
- AB_416326 (BioLegend Cat. No. 313502)
- Structure
- CD28/CTLA-4, 47-57 kD
- Distribution
-
Activated T cells, subset of thymocytes
- Function
- Costimulates T cell activation, proliferation, humoral immune response
- Ligand/Receptor
- B7h/B7RP-1/GL-50
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Redoglia V,?et al.?1996.?Eur. J. Immunol.?26:2781.
2. Hutloff A,?et al.?1999.?Nature?397:263.
3. Buonfiglio D,?et al.?2000.?Eur. J. Immunol.?30:3463.
4. Coyle AJ,?et al.?2000.?Immunity?13:95.
- Gene ID
- 100048841 View all products for this Gene ID 29851 View all products for this Gene ID 64545 View all products for this Gene ID
- UniProt
- View information about CD278 on UniProt.org
