PerCP/Cyanine5.5 anti-human/mouse/rat CD278 (ICOS)
- 产品名称：
- PerCP/Cyanine5.5 anti-human/mouse/rat CD278 (ICOS)
- 产品类别：
- 抗体
- 产品编号：
- 313517
- 产品应用：
- 313517
- Verified Reactivity
- Human, Mouse, Rat
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus, Pig
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse T cell clone D10.G4.1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
- The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1,?in vitro?costimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 313512).
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Redoglia V,?et al.?1996.?Eur. J. Immunol.?26:2781. (FC IP Costim)
- Yagi J,?et al.?2003.?J. Immunol.?171:783. (FC)
- Arimura Y,?et al.?2002.?Int. Immunol.?14:555. (Costim)
- Arimura Y,?et al.?2004.?J. Biol. Chem.?279:11408. (Costim)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
- Gordon E, et al. 2016. Proc Natl Acad Sci U S A. 113: 8765 - 8770. PubMed
- Pauthner MG, et al. 2019. Immunity. 50:241. PubMed
- Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
- Pauthner M et al. 2017. Immunity. 46(6):1073-1088 . PubMed
- Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
- Stewart JM, et al. 2020. ACS Biomater Sci Eng. 6:5941. PubMed
- Hasegawa M, et al. 2013. Rheumatology. 52:242. PubMed
- Knolle MD et al. 2018. Frontiers in immunology. 1.925 . PubMed
- Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
- Gomez-Rodriguez J, et al. 2016. Nat Commun. 7:10857. PubMed
- Van Gool F, et al. 2019. Immunity. 50:362. PubMed
- Colineau L, et al. 2015. PLoS One. 10: e0140978. PubMed
- Pilkinton MA, et al. 2017. Vaccine. 35:329. PubMed
- Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
- Vyas AK, et al. 2019. Hepatol Commun. 3:795. PubMed
- Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
- Mudd PA, et al. 2022. Cell. 185:603. PubMed
- Engel I, et al. 2016. Nat Immunol. 10.1038/ni.3437. PubMed
- Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
- Jie Z, et al. 2014. J Immunol. 192:3289. PubMed
- RRID
- AB_10639735 (BioLegend Cat. No. 313517) AB_10641280 (BioLegend Cat. No. 313518)
- Structure
- CD28/CTLA-4, 47-57 kD
- Distribution
-
Activated T cells, subset of thymocytes
- Function
- Costimulates T cell activation, proliferation, humoral immune response
- Ligand/Receptor
- B7h/B7RP-1/GL-50
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Redoglia V,?et al.?1996.?Eur. J. Immunol.?26:2781.
2. Hutloff A,?et al.?1999.?Nature?397:263.
3. Buonfiglio D,?et al.?2000.?Eur. J. Immunol.?30:3463.
4. Coyle AJ,?et al.?2000.?Immunity?13:95.
- Gene ID
- 100048841 View all products for this Gene ID 29851 View all products for this Gene ID 64545 View all products for this Gene ID
- UniProt
- View information about CD278 on UniProt.org
