PE/Cyanine7 anti-human/mouse/rat CD278 (ICOS) Anti
- 产品名称：
- PE/Cyanine7 anti-human/mouse/rat CD278 (ICOS) Anti
- 产品类别：
- 抗体
- 产品编号：
- 313519
- 产品应用：
- 313519
- Verified Reactivity
- Human, Mouse, Rat
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus, Pig
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse T cell clone D10.G4.1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
- The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1,?in vitro?costimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 313512).
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Redoglia V,?et al.?1996.?Eur. J. Immunol.?26:2781. (FC IP Costim)
- Yagi J,?et al.?2003.?J. Immunol.?171:783. (FC)
- Arimura Y,?et al.?2002.?Int. Immunol.?14:555. (Costim)
- Arimura Y,?et al.?2004.?J. Biol. Chem.?279:11408. (Costim)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
- Oh–Hora M, et al. 2019. J Immunol. 202:2616. PubMed
- Vono M, et al. 2020. Cell Reports. 28(7):1773-1784.e5.. PubMed
- Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed
- Matsui K, et al. 2015. PLoS One. 10: 0137195. PubMed
- Myers LM, et al. 2019. Nat Commun. 10:794. PubMed
- Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
- Liu R, et al. 2017. J Neuroimmunol. 10.1016/j.jneuroim.2017.08.005. PubMed
- Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed
- Gagne M, et al. 2022. Cell. 185:113. PubMed
- Sun Z, et al. 2021. eLife. 10:00. PubMed
- Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
- Miles B, et al. 2015. Nat Commun. 6: 8608. PubMed
- Herati R, et al. 2014. J Immunol. 193:3528. PubMed
- Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
- Shen X, et al. 2021. Front Immunol. 12:710750. PubMed
- Berezhnoy A, et al. 2020. Cell Rep Med. 1:100163. PubMed
- Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
- Zeis P, et al. 2020. Immunity. 53:775. PubMed
- Barbet G, et al. 2018. Immunity. 48:584. PubMed
- DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
- RRID
- AB_10641839 (BioLegend Cat. No. 313519) AB_10643411 (BioLegend Cat. No. 313520)
- Structure
- CD28/CTLA-4, 47-57 kD
- Distribution
-
Activated T cells, subset of thymocytes
- Function
- Costimulates T cell activation, proliferation, humoral immune response
- Ligand/Receptor
- B7h/B7RP-1/GL-50
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Redoglia V,?et al.?1996.?Eur. J. Immunol.?26:2781.
2. Hutloff A,?et al.?1999.?Nature?397:263.
3. Buonfiglio D,?et al.?2000.?Eur. J. Immunol.?30:3463.
4. Coyle AJ,?et al.?2000.?Immunity?13:95.
- Gene ID
- 100048841 View all products for this Gene ID 29851 View all products for this Gene ID 64545 View all products for this Gene ID
- UniProt
- View information about CD278 on UniProt.org
