GoInVivo™ Purified anti-human/mouse/rat CD27-上海雅吉生物科技有限公司

规格	价格	库存
5mg	￥ 9240	1

Product Details

Verified Reactivity

Human, African Green, Baboon, Cynomolgus, Mouse, Rat, Rhesus, Swine (Pig, Porcine)

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Mouse T cell clone D10.G4.1

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <1.0 EU/mg of the protein. (<0.1 pg/?g of the protein) as determined by the LAL test.

Preparation

The GoInVivo? antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by?immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

For?in vivo?and?in vitro?applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.

Application Notes

GoInVivo? products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the?GoInVivo? Webpage.

The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation¹ and in vitro costimulation of T cell activation^1,3,4. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 313512).

Application References (PubMed link indicates BioLegend citation)

Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. (FC IP Costim)
Yagi J, et al. 2003. J. Immunol. 171:783. (FC)
Arimura Y, et al. 2002. Int. Immunol. 14:555. (Costim)
Arimura Y, et al. 2004. J. Biol. Chem. 279:11408. (Costim)

RRID

AB_2728259 (BioLegend Cat. No. 313541) AB_2728260 (BioLegend Cat. No. 313542) AB_2728261 (BioLegend Cat. No. 313543) AB_2728262 (BioLegend Cat. No. 313544) AB_2728263 (BioLegend Cat. No. 313545) AB_2728264 (BioLegend Cat. No. 313546)

Antigen Details

Structure: CD28/CTLA-4, 47-57 kD
Distribution: Activated T cells, subset of thymocytes
Function: Costimulates T cell activation, proliferation, humoral immune response
Ligand/Receptor: B7h/B7RP-1/GL-50
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781.
2. Hutloff A, et al. 1999. Nature 397:263.
3. Buonfiglio D, et al. 2000. Eur. J. Immunol. 30:3463.
4. Coyle AJ, et al. 2000. Immunity 13:95.
Gene ID: 100048841 View all products for this Gene ID 29851 View all products for this Gene ID 64545 View all products for this Gene ID
UniProt: View information about CD278 on UniProt.org

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