Brilliant Violet 785™ anti-human/mouse/rat C
- 产品名称：
- Brilliant Violet 785™ anti-human/mouse/rat C
- 产品类别：
- 抗体
- 产品编号：
- 313533
- 产品应用：
- 313533
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2052 |
1 |
- Verified Reactivity
- Human, Mouse, Rat
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus, Pig
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse T cell clone D10.G4.1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785? under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
Brilliant Violet 785? excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
- The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1,?in vitro?costimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 313512).
- Application References
(PubMed link indicates BioLegend citation) -
- Redoglia V,?et al.?1996.?Eur. J. Immunol.?26:2781. (FC IP Costim)
- Yagi J,?et al.?2003.?J. Immunol.?171:783. (FC)
- Arimura Y,?et al.?2002.?Int. Immunol.?14:555. (Costim)
- Arimura Y,?et al.?2004.?J. Biol. Chem.?279:11408. (Costim)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Blache U, et al. 2021. Front Immunol. 12:658314. PubMed
- Marangoni F, et al. 2021. Cell. . PubMed
- Verma A, et al. 2021. Cell Rep. 37:109942. PubMed
- Zeis P, et al. 2020. Immunity. 53:775. PubMed
- Herati RS, et al. 2022. Nat Immunol. 23:1183. PubMed
- Baja?a S, et al. 2022. iScience. 25:103732. PubMed
- Field CS, et al. 2020. Cell Metab. 31:422. PubMed
- RRID
- AB_2629728 (BioLegend Cat. No. 313533) AB_2629729 (BioLegend Cat. No. 313534)
- Structure
- CD28/CTLA-4, 47-57 kD
- Distribution
-
Activated T cells, subset of thymocytes
- Function
- Costimulates T cell activation, proliferation, humoral immune response
- Ligand/Receptor
- B7h/B7RP-1/GL-50
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Redoglia V,?et al.?1996.?Eur. J. Immunol.?26:2781.
2. Hutloff A,?et al.?1999.?Nature?397:263.
3. Buonfiglio D,?et al.?2000.?Eur. J. Immunol.?30:3463.
4. Coyle AJ,?et al.?2000.?Immunity?13:95.
- Gene ID
- 100048841 View all products for this Gene ID 29851 View all products for this Gene ID 64545 View all products for this Gene ID
- UniProt
- View information about CD278 on UniProt.org
