APC anti-human/mouse/rat CD278 (ICOS) Antibody
- 产品名称：
- APC anti-human/mouse/rat CD278 (ICOS) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 313509
- 产品应用：
- 313509
- Verified Reactivity
- Human, Mouse, Rat
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus, Pig
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse T cell clone D10.G4.1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that reagents be titrated for optimal performance in the particular application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
- The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1,?in vitro?costimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 313512).
- Application References
(PubMed link indicates BioLegend citation) -
- Redoglia V,?et al.?1996.?Eur. J. Immunol.?26:2781. (FC IP Costim)
- Yagi J,?et al.?2003.?J. Immunol.?171:783. (FC)
- Arimura Y,?et al.?2002.?Int. Immunol.?14:555. (Costim)
- Arimura Y,?et al.?2004.?J. Biol. Chem.?279:11408. (Costim)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Wijesinghe A, et al. 2020. J Biomed Sci. 27:50:00. PubMed
- Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed
- Karim F, et al. 2021. Elife. 10:. PubMed
- Miles B, et al. 2015. Nat Commun. 6: 8608. PubMed
- Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
- Court AC, et al. 2020. EMBO Rep. 21:e48052. PubMed
- Zhang Y, et al. 2021. J Immunol Res. 2021:5599439. PubMed
- Cassotta A, et al. 2020. J Exp Med. 217:00:00. PubMed
- Sefik E, et al. 2021. Nat Biotechnol. . PubMed
- Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
- Zhang SL, et al. 2022. Gut Microbes. 14:2046246. PubMed
- Bonnet B, et al. 2016. J Immunol. 197: 188 - 198. PubMed
- Paul S, et al. 2021. Sci Transl Med. 13:. PubMed
- Llibre A, et al. 2016. J Immunol. 196: 2085 - 2094. PubMed
- NULL, et al. 2022. Cell. 185:916. PubMed
- Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
- Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
- Lim S, et al. 2017. Sci Rep. 7:40040. PubMed
- Deakin A, et al. 2014. PLoS One. 9:107490. PubMed
- Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
- Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
- Cassotta A, et al. 2020. Eur J Immunol. . PubMed
- RRID
- AB_416333 (BioLegend Cat. No. 313509) AB_416334 (BioLegend Cat. No. 313510)
- Structure
- CD28/CTLA-4, 47-57 kD
- Distribution
-
Activated T cells, subset of thymocytes
- Function
- Costimulates T cell activation, proliferation, humoral immune response
- Ligand/Receptor
- B7h/B7RP-1/GL-50
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Redoglia V,?et al.?1996.?Eur. J. Immunol.?26:2781.
2. Hutloff A,?et al.?1999.?Nature?397:263.
3. Buonfiglio D,?et al.?2000.?Eur. J. Immunol.?30:3463.
4. Coyle AJ,?et al.?2000.?Immunity?13:95.
- Gene ID
- 100048841 View all products for this Gene ID 29851 View all products for this Gene ID 64545 View all products for this Gene ID
- UniProt
- View information about CD278 on UniProt.org
