Alexa Fluor® 647 anti-human/mouse/rat CD278 (I
- 产品名称：
- Alexa Fluor® 647 anti-human/mouse/rat CD278 (I
- 产品类别：
- 抗体
- 产品编号：
- 313515
- 产品应用：
- 313515
- Verified Reactivity
- Human, Mouse, Rat
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus, Pig
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse T cell clone D10.G4.1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
- The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1,?in vitro?costimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 313512).
- Application References
(PubMed link indicates BioLegend citation) -
- Redoglia V,?et al.?1996.?Eur. J. Immunol.?26:2781. (FC IP Costim)
- Yagi J,?et al.?2003.?J. Immunol.?171:783. (FC)
- Arimura Y,?et al.?2002.?Int. Immunol.?14:555. (Costim)
- Arimura Y,?et al.?2004.?J. Biol. Chem.?279:11408. (Costim)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
- Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
- Lu K, et al. 2015. J Leukoc Biol. 98: 301-311. PubMed
- McPhee C, et al. 2011. J Immunol. 187:4695. PubMed
- RRID
- AB_2122583 (BioLegend Cat. No. 313515) AB_2122582 (BioLegend Cat. No. 313516)
- Structure
- CD28/CTLA-4, 47-57 kD
- Distribution
-
Activated T cells, subset of thymocytes
- Function
- Costimulates T cell activation, proliferation, humoral immune response
- Ligand/Receptor
- B7h/B7RP-1/GL-50
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Redoglia V,?et al.?1996.?Eur. J. Immunol.?26:2781.
2. Hutloff A,?et al.?1999.?Nature?397:263.
3. Buonfiglio D,?et al.?2000.?Eur. J. Immunol.?30:3463.
4. Coyle AJ,?et al.?2000.?Immunity?13:95.
- Gene ID
- 100048841 View all products for this Gene ID 29851 View all products for this Gene ID 64545 View all products for this Gene ID
- UniProt
- View information about CD278 on UniProt.org
