PerCP/Cyanine5.5 anti-mouse CD45RB Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD45RB Antibody
- 产品类别：
- 抗体
- 产品编号：
- 103313
- 产品应用：
- 103313
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Cloned mouse Th2 cell lines
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Bottomly K, et al. 1989. Eur. J. Immunol. 19:617.
- Norian LA and Allen PM. 2004. J. Immunol. 173:835.
- Product Citations
-
- Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
- RRID
- AB_1953290 (BioLegend Cat. No. 103313) AB_2284707 (BioLegend Cat. No. 103314)
- Structure
- Phosphatase receptor (CD45 gene exon 5), 220 kD
- Distribution
-
Peripheral B cells, na?ve T cells, most thymocytes, low density on macrophages and dendritic cells
- Function
- Phosphatase, T cell development and activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4, Thy1
- Cell Type
- B cells, Dendritic cells, Macrophages, T cells, Thymocytes, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45RB on UniProt.org
