PE anti-mouse CD45RB Antibody
- 产品名称：
- PE anti-mouse CD45RB Antibody
- 产品类别：
- 抗体
- 产品编号：
- 103307
- 产品应用：
- 103307
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Cloned mouse Th2 cell lines
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Bottomly K, et al. 1989. Eur. J. Immunol. 19:617.
- Norian LA and Allen PM. 2004. J. Immunol. 173:835.
- Product Citations
-
- Lyons J, et al. 2018. PLoS Biol. 16:e2002417. PubMed
- Yu Y, et al. 2022. iScience. 25:105004. PubMed
- Súarez-López L, et al. 2021. iScience. 24:103406. PubMed
- Shao B, et al. 2019. J Immunol. 10.4049/jimmunol.1900680. PubMed
- Lyons J, et al. 2018. Sci Signal. 11. PubMed
- Fujiwara H, et al. 2021. Nat Immunol. 22:1440. PubMed
- Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed
- Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
- Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
- Hao C, et al. 2021. Frontiers in Cell and Developmental Biology. 9:695015. PubMed
- Imbratta C, et al. 2019. Sci Rep. 9:6135. PubMed
- Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
- Gonzalez MM, et al. 2021. J Clin Invest. 131:. PubMed
- RRID
- AB_313014 (BioLegend Cat. No. 103307) AB_313015 (BioLegend Cat. No. 103308)
- Structure
- Phosphatase receptor (CD45 gene exon 5), 220 kD
- Distribution
-
Peripheral B cells, na?ve T cells, most thymocytes, low density on macrophages and dendritic cells
- Function
- Phosphatase, T cell development and activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4, Thy1
- Cell Type
- B cells, Dendritic cells, Macrophages, T cells, Thymocytes, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45RB on UniProt.org
