Pacific Blue™ anti-mouse CD45RB Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1548	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Cloned mouse Th2 cell lines

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 ?g per 10⁶ cells in 100 ?l volume or 100 ?l of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.

Application References (PubMed link indicates BioLegend citation)

Bottomly K, et al. 1989. Eur. J. Immunol. 19:617.
Norian LA and Allen PM. 2004. J. Immunol. 173:835.

Product Citations

Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
Wilfahrt D, et al. 2021. Elife. 10:. PubMed

RRID

AB_2174406 (BioLegend Cat. No. 103315) AB_2174405 (BioLegend Cat. No. 103316)

Antigen Details

Structure: Phosphatase receptor (CD45 gene exon 5), 220 kD
Distribution: Peripheral B cells, na?ve T cells, most thymocytes, low density on macrophages and dendritic cells
Function: Phosphatase, T cell development and activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4, Thy1
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Thymocytes, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264 View all products for this Gene ID
UniProt: View information about CD45RB on UniProt.org

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