Purified anti-mouse IL-12 (p70) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1128	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

CHO expressed, recombinant mouse IL-12 p70

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12(p70) recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified C18.2 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with the biotinylated C17.8 antibody as detection antibody and recombinant mouse IL-12 p70 as the standard.

Product Citations

Volpedo G, et al. 2022. NPJ Vaccines. 7:32. PubMed
Liang D, et al. 2018. Front Immunol. 8:1801. PubMed
Zhan CS, et al. 2020. FASEB J. 14006:34. PubMed
Jin X, et al. 2020. Cell Reports. 31(8):107690. PubMed
Zhou Y, et al. 2018. Cell Mol Immunol. 15:246. PubMed
Liu C, et al. 2019. Theranostics. 9:1369. PubMed
Zhao L, et al. 2021. STAR Protocols. 2(3):100732. PubMed

RRID

AB_2123771 (BioLegend Cat. No. 511801) AB_2123769 (BioLegend Cat. No. 511802)

Antigen Details

Structure: heterodimeric cytokine composed of p40 subunit shared with IL-23 and a specific p35 subunit
Distribution: Secreted by dendritic cells, monocytes/macrophages, B cells, T cells
Function: Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Interaction: T cells, NK cells
Ligand/Receptor: IL-12Rβ1 binds p40 shared with IL-23; dimeric with IL-12Rβ2 binds p35
Bioactivity: IL-12 p70 (p40:p35) induces IFN-γ, TNF-α production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes.
Cell Type: Dendritic cells, Monocytes, Macrophages, B cells, T cells
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Lankford CS, et al. 2003 J. Leukoc. Biol.. 73:49.
2. Parham C, et al. 2002 J. Immunol.. 168:5699.
3. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Quesniaux V. 1992. Research Immunol. 143:385.
6. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
Gene ID: 16159 View all products for this Gene ID 16160 View all products for this Gene ID
UniProt: View information about IL-12 p70 on UniProt.org

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