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Purified anti-human C5a/C5a(desArg) Antibody
产品名称:
Purified anti-human C5a/C5a(desArg) Antibody
产品类别:
抗体
产品编号:
518201
产品应用:
518201
[价格]
规格 价格 库存
50µg ¥ 1212 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human C5a
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-8 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of C5a recombinant protein ranging from 15.6 to 1000 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The C17/5 antibody reacts with human C5a and C5a(desArg). C17/5 is specific for a C5 activation-dependent neo-epitope expressed only on C5a and C5a(desArg) but not C5, and has been shown to be an effective inhibitor of C5a.4

ELISA Capture: The purified C17/5 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated G25/2 antibody (Cat. No. 518306) as the detection antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Oppermann M, et al. 1991. Kidney Int. 40:939947.
  2. Oppermann M, et al. 1992. Immunopharm. 24:119134.
  3. Elsner J, et al. 1994. Blood 83:3324.
  4. Wittmann M, et al. 1999. J. Immunol. 162: 6763.
RRID
AB_11123910 (BioLegend Cat. No. 518201) AB_11124718 (BioLegend Cat. No. 518202)

Antigen Details

Biology Area
Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Lundwall AB, et al. 1985. J. Biol. Chem. 260:2108.
2. Fernandez HN, et al. 1978. J. Biol. Chem. 253:6955.
3. Tack BF, et al. 1979. Biochemistry 18:1490.

Gene ID
727 View all products for this Gene ID
UniProt
View information about C5a/C5adesArg/C5 on UniProt.org

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