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Ultra-LEAF™
产品名称：
Ultra-LEAF™
产品类别：
抗体
产品编号：
505307
产品应用：
505307
[价格]
规格 价格 库存
100µg ￥ 1452 1

产品详情

Product Details

Verified Reactivity
Mouse, IL-12/IL-23 p40 subunit (monomer, homodimer and heterodimer IL-12 p35/p40 or IL-23 p19/p40)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO expressed, recombinant mouse IL-12 p70
Formulation
0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
Application

ELISA - Quality tested
Neut, ICFC, IP, WB - Reported in the literature, not verified in house

?
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection1,2,6-9 or ELISPOT Detection: The biotinylated C17.8 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified C15.6 antibody (Cat. No. 505202/505208) as capture antibody and recombinant mouse IL-12/IL-23 p40 (Cat. No. 576909) or recombinant mouse IL-12 p70 (Cat. No. 577009) as the standard.
Neutralization1,2,4,5,6,10: The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse IL-12 bioactivity in vivo and in vitro (Cat. No. 505304). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 505308) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).
Additional reported applications (for relevant formats of this clone) include: Western blotting3, immunoprecipitation3, and intracellular immunofluorescent staining and flow cytometric analysis.
Note: For testing mouse IL-12 p40 (monomer, dimer, heterodimer) in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 431601 to 431606) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
  2. Reichmann G, et al. 1999. J. Immunol. 163:3354.
  3. Wysocka M, et al. 1995. Eur. J. Immunol. 25:672.
  4. Neucath M, et al. 1995. J. Exp. Med. 182:1281.
  5. Cousens LP, et al. 1999 J. Exp. Med. 189:1315.
  6. Gately M. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.16.
  7. O'Connell PJ, et al. 2006. Blood 107:1010.
  8. Dzhagalov I, et al. 2007. J. Immunol. 178:2113.
  9. Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
  10. Sugimoto K, et al. 2007. Gastroenterology 133:124. PubMed
Product Citations
  1. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
RRID
AB_11150770 (BioLegend Cat. No. 505307) AB_11148948 (BioLegend Cat. No. 505308) AB_2783330 (BioLegend Cat. No. 505309) AB_2783331 (BioLegend Cat. No. 505310) AB_2783332 (BioLegend Cat. No. 505311) AB_2783333 (BioLegend Cat. No. 505312)

Antigen Details

Structure
Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity
IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me
Cell Sources
Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets
T cells, NK cells
Receptors
IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Oppmann B, et al. 2000 Immunity. 13:715.
6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002 J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002 J. Immunol. 168:5448.
9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49.

Regulation
Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Gene ID
16160 View all products for this Gene ID
UniProt
View information about IL-12 p40 on UniProt.org

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