Alexa Fluor® 700 anti-mouse IL-12/IL-23 p40 An-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1836	1

Product Details

Verified Reactivity

Mouse, IL-12/IL-23 p40 subunit (monomer, homodimer and heterodimer IL-12 p35/p40 or IL-23 p19/p40)

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

CHO-expressed, recombinant mouse IL-12 p70

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

ELISA or ELISPOT Capture^1,2,4,6,8,10: The purified C15.6 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated C17.8 (Cat. No. 505302) antibody as the detecting antibody. This assay detects p40 as monomer, homodimer, or heterodimer complexed with p35. The LEAF? purified antibody is suggested for ELISPOT capture.
Additional reported applications (for the relevant formats) include: immunohistochemical staining⁹ of paraformaldehyde-fixed saponin-treated frozen tissue sections, immunoprecipitation³, and Western blotting³.
Note: For testing mouse IL-12 p40 (monomer, dimer, heteromer) in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 431601 to 431606) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
Reichmann G, et al. 1999. J. Immunol. 163:3354.
Wysocka M, et al. 1995. Eur. J. Immunol. 25:672.
Gately M. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.16.
Macatonia S, et al. 1995. J. Immunol. 154:5071.
O'Connell PJ, et al. 2006. Blood 107:1010.
Akilov OE, et al. 2007. J. Leukocyte Biol. 2007;10.1189/jlb.0706439.
Dzhagalov I, et al. 2007. J. Immunol. 178:2113.
Huang LY, et al. 2001. J. Immunol. 167:1423.
Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
Malu DT, et al. 2011. J. Immunol. 186:6271. PubMed

RRID

AB_2894642 (BioLegend Cat. No. 505213) AB_2894642 (BioLegend Cat. No. 505214)

Antigen Details

Structure: Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity: IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of T_H1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me
Cell Sources: Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets: T cells, NK cells
Receptors: IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Oppmann B, et al. 2000 Immunity. 13:715.
6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002 J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002 J. Immunol. 168:5448.
9. Lankford, CS, et al. 2003 J. Leukocyte Biol. 73:49.
Regulation: Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Gene ID: 16160 View all products for this Gene ID
UniProt: View information about IL-12 p40 on UniProt.org

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