Ultra-LEAF™ Purified anti-human IL-3 Antibod
- 产品名称：
- Ultra-LEAF™ Purified anti-human IL-3 Antibod
- 产品类别：
- 抗体
- 产品编号：
- 500506
- 产品应用：
- 500506
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Yeast-expressed, recombinant human IL-3
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
IP, WB - Reported in the literature, not verified in house
- Recommended Usage
Ultra-LEAF? Purified anti-human IL-3 antibody inhibits the proliferation of TF-1 erythroleukemic cells induced by Recombinant Human IL-3 (carrier-free) (7 ng/ml) in a dose dependent manner, with an ED50 = 0.02 – 0.1 ?g/ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA1-4 or ELISPOT Capture: The purified BVD8-3G11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD3-1F9 antibody (Cat. No. 500604) as the detecting antibody.
Additional reported applications include: Western blotting4 and immunoprecipitation4.
- Application References
(PubMed link indicates BioLegend citation) -
- Abrams JS, et al. 1992. Immunol. Rev. 127:5. (ELISA)
- Abrams JS et al. 1994. Eosnophil-active cytokines in human disease: development and use of monoclonal antibodies to il-3, il-5, and GM-CSF. In Eosinophils in Allergy and Inflammation. Gleich GJ and Kay AB, eds. Marcel Dekker, Inc. New York, NY. p. 133. (ELISA)
- Abrams J. 1995. Curr. Prot. Immunol. 6.20. (ELISA)
- Kaushansky K. 1992. J. Clin. Invest. 90:1879. (ELISA, IP, WB)
- Mahanty S, et al. 1992. J. Immunol. 148:3567.
- RRID
- AB_2616770 (BioLegend Cat. No. 500506)
- Structure
- Cytokine; 14-30 kD (Mammalian)
- Bioactivity
- Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase.
- Cell Sources
- Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells.
- Cell Targets
- Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages.
- Receptors
- IL-3R, α subunit (CD123), common β subunit (CDw131).
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Frendl G. 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle J. 1992. Chem. Immunology 51:65.
- Regulation
- Upregulated by T cell stimulation; downregulated by glucocorticoids.
- Gene ID
- 3562 View all products for this Gene ID
- UniProt
- View information about IL-3 on UniProt.org
