PE anti-human IL-3 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100tests	￥ 3648	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Yeast-expressed, recombinant human IL-3

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

ELISA^1-3,6 or ELISPOT⁴ Detection: The biotinylated BVD3-1F9 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified BVD8-3G11 (Cat. No. 500502) antibody as the capture antibody.
Flow Cytometry: The fluorochrome-labelled BVD3-1F9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-3 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Additional reported applications (for the relevant formats) include: immunoprecipitation⁶, Western blotting⁶, neutralization^1,6, immunohistochemical staining^5,7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.

Application References (PubMed link indicates BioLegend citation)

Abrams J, et al. 1992. Immunological Reviews 127:5.
Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133.
Abrams J. 1995. Curr. Prot. Immunol.. 6.20.
Mahanty S, et al. 1992. J. Immunol. 148:3567.
Andersson U, et al. 1993. Detection and quantification of gene expression. New York: Springer-Verlag.
Kaushansky K. 1992. J. Clin. Invest. 90:1879.
Andersson J, et al. 1994. Immunology 83:16.

RRID

AB_2123712 (BioLegend Cat. No. 500606)

Antigen Details

Structure: Cytokine; 14-30 kD (Mammalian)
Bioactivity: Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase
Cell Sources: Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells
Cell Targets: Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
Receptors: IL-3R, α subunit (CD123), common β subunit (CDw131)
Biology Area: Cell Biology, Stem Cells
Molecular Family: Growth Factors, Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Frendl G. 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle J. 1992. Chem. Immunology 51:65.
Regulation: Upregulated by T cell stimulation; downregulated by glucocorticoids
Gene ID: 3562 View all products for this Gene ID
UniProt: View information about IL-3 on UniProt.org

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