Biotin anti-human IL-3 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
500µg	￥ 3936	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Yeast-expressed, recombinant human IL-3

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-3 protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA^1-3,6 or ELISPOT⁴ Detection: The biotinylated BVD3-1F9 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified BVD8-3G11 (Cat. No. 500502) antibody as the capture antibody.
Flow Cytometry: The fluorochrome-labelled BVD3-1F9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-3 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Additional reported applications (for the relevant formats) include: immunoprecipitation⁶, Western blotting⁶, neutralization^1,6, immunohistochemical staining^5,7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.

Application References (PubMed link indicates BioLegend citation)

Abrams J, et al. 1992. Immunological Reviews 127:5.
Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133.
Abrams J. 1995. Curr. Prot. Immunol.. 6.20.
Mahanty S, et al. 1992. J. Immunol. 148:3567.
Andersson U, et al. 1993. Detection and quantification of gene expression. New York: Springer-Verlag.
Kaushansky K. 1992. J. Clin. Invest. 90:1879.
Andersson J, et al. 1994. Immunology 83:16.

RRID

AB_2123713 (BioLegend Cat. No. 500604)

Antigen Details

Structure: Cytokine; 14-30 kD (Mammalian)
Bioactivity: Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase
Cell Sources: Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells
Cell Targets: Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
Receptors: IL-3R, α subunit (CD123), common β subunit (CDw131)
Biology Area: Cell Biology, Stem Cells
Molecular Family: Growth Factors, Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Frendl G. 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle J. 1992. Chem. Immunology 51:65.
Regulation: Upregulated by T cell stimulation; downregulated by glucocorticoids
Gene ID: 3562 View all products for this Gene ID
UniProt: View information about IL-3 on UniProt.org

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