Purified anti-human GM-CSF (Maxpar® Ready) Ant-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2052	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli-expressed, recombinant human GM-CSF.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA^1-4or ELISPOT^3,4Detection, Neutralization¹, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated^5,6and acetone-fixed⁷ frozen tissue sections, and immunocytochemistry.

Note:?For testing human GM-CSF in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 432001 to 432006) are specially developed and recommended.

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut, IP)
Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133. (ELISA)
Bacchetta R, et al. 1990. J. Immunol. 144:902. (ELISA)
Kita H, et al. 1991. J. Exp. Med. 174:745. (ELISA)
Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
Andersson J, et al. 1994. Immunology 83:16. (IHC)
Rasouli J, et al. 2015. J. Immunol. 11:5085-93. (IHC)

RRID

AB_2563770 (BioLegend Cat. No. 502315)

Antigen Details

Structure: Cytokine; 22 kD (Mammalian)
Bioactivity: Growth/development factor for granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Cell Sources: T cells, macrophages, fibroblasts, endothelial cells, mast cells
Cell Targets: Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Receptors: Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131), which is also shared with the IL-3 and IL-5 receptor α chains
Biology Area: Cell Biology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Demetri G, et al. 1991. Blood 78:2791.
3. Fan D, et al. 1991. In vivo 5:571.
4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.
Regulation: Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Gene ID: 1437 View all products for this Gene ID
UniProt: View information about GM-CSF on UniProt.org

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