PE/Dazzle™ 594 anti-human GM-CSF Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1980	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli-expressed, recombinant human GM-CSF.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: ELISA^1-4or ELISPOT^3,4Detection, Neutralization¹, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated^5,6and acetone-fixed⁷ frozen tissue sections, and immunocytochemistry.

Note:?For testing human GM-CSF in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 432001 to 432006) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut, IP)
Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133. (ELISA)
Bacchetta R, et al. 1990. J. Immunol. 144:902. (ELISA)
Kita H, et al. 1991. J. Exp. Med. 174:745. (ELISA)
Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
Andersson J, et al. 1994. Immunology 83:16. (IHC)
Rasouli J, et al. 2015. J. Immunol. 11:5085-93. (IHC)

Product Citations

Affandi AJ, et al. 2018. Eur J Immunol. 48:522. PubMed

RRID

AB_2616930 (BioLegend Cat. No. 502317) AB_2616931 (BioLegend Cat. No. 502318)

Antigen Details

Structure: Cytokine; 22 kD (Mammalian)
Bioactivity: Growth/development factor for granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Cell Sources: T cells, macrophages, fibroblasts, endothelial cells, mast cells
Cell Targets: Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Receptors: Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131), which is also shared with the IL-3 and IL-5 receptor α chains
Biology Area: Cell Biology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Demetri G, et al. 1991. Blood 78:2791.
3. Fan D, et al. 1991. In vivo 5:571.
4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.
Regulation: Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Gene ID: 1437 View all products for this Gene ID
UniProt: View information about GM-CSF on UniProt.org

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