Biotin anti-human GM-CSF Antibody -上海雅吉生物科技有限公司

规格	价格	库存
500µg	￥ 3936	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli-expressed, recombinant human GM-CSF.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection, ELISPOT Detection, ICFC

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of human GM-CSF protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA^1-4or ELISPOT^3,4Detection, Neutralization¹, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated^5,6and acetone-fixed⁷ frozen tissue sections, and immunocytochemistry.

Note:?For testing human GM-CSF in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 432001 to 432006) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut, IP)
Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133. (ELISA)
Bacchetta R, et al. 1990. J. Immunol. 144:902. (ELISA)
Kita H, et al. 1991. J. Exp. Med. 174:745. (ELISA)
Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
Andersson J, et al. 1994. Immunology 83:16. (IHC)
Rasouli J, et al. 2015. J. Immunol. 11:5085-93. (IHC)

Product Citations

Loudon P, et al. 2010. PLoS One. 5:e11021. PubMed
Gallo R, et al. 2012. J Immunol. 189:1689. PubMed

RRID

AB_315218 (BioLegend Cat. No. 502304)

Antigen Details

Structure: Cytokine; 22 kD (Mammalian)
Bioactivity: Growth/development factor for granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Cell Sources: T cells, macrophages, fibroblasts, endothelial cells, mast cells
Cell Targets: Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Receptors: Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131), which is also shared with the IL-3 and IL-5 receptor α chains
Biology Area: Cell Biology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Demetri G, et al. 1991. Blood 78:2791.
3. Fan D, et al. 1991. In vivo 5:571.
4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.
Regulation: Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Gene ID: 1437 View all products for this Gene ID
UniProt: View information about GM-CSF on UniProt.org

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