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Biotin anti-human G-CSF Antibody
产品名称:
Biotin anti-human G-CSF Antibody
产品类别:
抗体
产品编号:
506702
产品应用:
506702
[价格]
规格 价格 库存
500µg ¥ 3936 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant human G-CSF
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1-4 ?g/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Detection1,2: The biotinylated BVD11-37G10 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified BVD13-3A5 antibody (Cat. No. 502102/502104) as the capture antibody.
Additional reported applications (for the relevant formats) include: Western blotting, neutralization1,2, and immunohistochemistry3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1995. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.20
  2. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  3. Andersson J, et al. 1994. Immunol. 83:16.
  4. Sander B, et al. 1991. Immunol. Rev. 119:65.
RRID
AB_2083731 (BioLegend Cat. No. 506702)

Antigen Details

Structure
Cytokine; 21 kD (Mammalian)
Bioactivity
Growth/differentiation/activating factor for neutrophils; proliferation/migration endothelial cells cells; synergistic with IL-3 growth of hematopoietic progenitors
Cell Sources
Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, bone marrow stromal cells
Cell Targets
Hematopoietic progenitors of neutrophil/granulocyte lineage, endothelial cells
Receptors
CD114 (G-CSFR)
Cell Type
Neutrophils
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Demetri G, et al. 1991. Blood 78:2791.
3. Moore M. 1991. Annu. Rev. Immunol. 9:159.
4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.

Regulation
Upregulated by IL-1, IL-17, TNF-α, IFN-γ, GM-CSF; downregulated by prostaglandin E2
Gene ID
1441 View all products for this Gene ID
UniProt
View information about G-CSF on UniProt.org

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