Ultra-LEAF? Purified anti-human IL-7 Antibody
- 产品名称：
- Ultra-LEAF? Purified anti-human IL-7 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 501305
- 产品应用：
- 501305
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli - expressed, recombinant human IL-7
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
ELISA Capture - Quality tested
ELISPOT Capture, Neut, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by?ELISA assay. For ELISA or ELISPOT capture applications, a concentration range of 2.0 - 6.0 ?g/mL?is recommended. To obtain a linear standard curve, serial dilutions of IL-7 recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA or ELISPOT Capture1,2: The purified BVD10-40F6 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-7 antibody (Cat. No. 506602) as the detecting antibody. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture.
Neutralization1: The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-7 bioactivity (Cat. No. 501305 & 501306).
Additional reported applications (for the relevant formats) include: Western blotting.
- Application References
(PubMed link indicates BioLegend citation) -
- Abrams J, et al. 1992. Immunol. Rev. 127:5.
- Abrams J. 1995. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.20.
- RRID
- AB_2861028 (BioLegend Cat. No. 501305) AB_2861029 (BioLegend Cat. No. 501306)
- Structure
- Cytokine; 20-28 kD (Mammalian)
- Bioactivity
- Proliferation of pre-/pro-B cells, thymocytes, T cells; formation of LAK cells, CTLs, stimulation of tumoricidal activity monocytes/macrophages; upregulate proinflammatory cytokines in myeloid cells
- Cell Sources
- Adherent bone marrow stromal cells, thymic stromal cells, spleen, keratinocytes
- Cell Targets
- Progenitor B and T cells; CD4-CD8- thymocytes; megakaryocytes
- Receptors
- Heterodimer IL-7Rα (CD127); γ-subunit (CD132) in common with IL-2R, IL-4R, IL-13R, IL-15R
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Park L, et al. 1992. Adv. Exp. Med. Biol. 323 125.
3. Melchers F, et al. 1992. Adv. Exp. Med. Biol. 323 111.
- Regulation
- Downregulated by TGF-β
- Gene ID
- 3574 View all products for this Gene ID
- UniProt
- View information about IL-7 on UniProt.org
