Biotin anti-human IL-7 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1416	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli - expressed, recombinant human IL-7

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 1-4 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-7 recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 2-8 ?g/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Detection: The biotinylated BVD10-11C10 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified BVD10-40F6 antibody (Cat. No. 501302/501304) as the capture antibody.
Additional reported applications (for the relevant formats) include: The BVD10-11C10 is a non-neutralizing antibody.

Application References (PubMed link indicates BioLegend citation)

Abrams J, et al. 1992. Immunol. Rev. 127:5.
Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons New York.

Product Citations

Haug V, et al. 2020. Transplantation. e260:104. PubMed
Gilboa T, et al. 2020. Adv Healthc Mater. e2001111:. PubMed

RRID

AB_2296031 (BioLegend Cat. No. 506601) AB_2280326 (BioLegend Cat. No. 506602)

Antigen Details

Structure: Cytokine; 20-28 kD (Mammalian)
Bioactivity: Proliferation of pre-/pro-B cells, thymocytes, T cells; formation of LAK cells, CTLs, stimulation of tumoricidal activity monocytes/macrophages; upregulate proinflammatory cytokines in myeloid cells
Cell Sources: Adherent bone marrow stromal cells, thymic stromal cells, spleen, keratinocytes
Cell Targets: Progenitor B and T cells; CD4^-CD8^- thymocytes; megakaryocytes
Receptors: Heterodimer IL-7Rα (CD127); γ-subunit (CD132) in common with IL-2R, IL-4R, IL-13R, IL-15R
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Park L, et al. 1992. Adv. Exp. Med. Biol. 323 125.
3. Melchers F, et al. 1992. Adv. Exp. Med. Biol. 323 111.
Regulation: Downregulated by TGF-β
Gene ID: 3574 View all products for this Gene ID
UniProt: View information about IL-7 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们