PerCP/Cyanine5.5 anti-human CD144 (VE-Cadherin) An-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1764	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone BV9 has been shown to block VE-cadherin, causing a redistribution of VE-cadherin away from intracellular junctions.⁶ This clone binds to EC3-EC4 region in the extracellular domain of human VE-cadherin.⁷ Additional reported applications (for the relevant formats) include: Western Blotting^1,2, immunofluorescence microscopy^1,3, immunoprecipitation^1,4, blocking angiogenesis in vitro^4,5, inhibiting VE-cadherin reorganization⁴, and inducing endothelial cell apoptosis⁴.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Almagro S, et al. 2010. Mol. Cell Biol. 30:1703. (WB, IF, IP)
Zhang F, et al. 2004. J. Biol. Chem. 279:11760. (WB)
Iurlaro M, et al. 2004. Am. J. Pathol. 165:181. (IF)
Corada M, et al. 2001. Blood 97:1679. (IP, Block)
Kooistra M, et al. 2005. FEBS 579:4966. (Block)
Corada M, et al. 2001. Blood 97:1679. (Block)
Bouillet L, et al. 2013. Laboratory Investigation 93:1194-11202.

Product Citations

Sierra-Parraga JM, et al. 2020. Stem Cell Res Ther. 0.702777778. PubMed

RRID

AB_2562927 (BioLegend Cat. No. 348509) AB_2562928 (BioLegend Cat. No. 348510)

Antigen Details

Structure: Member of the cadherin family; calcium-dependent transmembrane cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region
Distribution: Endothelial cells, hematopoietic stem cells, perineurial cells
Function: Mediates calcium-dependent homophilic cell adhesion, plays fundamental roles in microvascular permeability and in the morphogenic and proliferative events associated with angiogenesis
Ligand/Receptor: VE-cadherin
Cell Type: Endothelial cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
Antigen References: 1. Taddei A, et al. 2008. Nat. Cell Biol. 10:923.
2. Gavard J, et al. 2006. Nat. Cell Biol. 8:1223.
3. Kim I, et al. 2005. Blood 106:903.
4. Suzuki S, et al. 1991. Cell Regul. 2:261.
Gene ID: 1003 View all products for this Gene ID
UniProt: View information about CD144 on UniProt.org

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