Alexa Fluor® 647 anti-human CD144 (VE-Cadherin-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1488	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Clone BV9 has been shown to block VE-cadherin, causing a redistribution of VE-cadherin away from intracellular junctions.⁶ This clone binds to EC3-EC4 region in the extracellular domain of human VE-cadherin.⁷ Additional reported applications (for the relevant formats) include: Western Blotting^1,2, immunofluorescence microscopy^1,3, immunoprecipitation^1,4, blocking angiogenesis in vitro^4,5, inhibiting VE-cadherin reorganization⁴, and inducing endothelial cell apoptosis⁴.

Application References (PubMed link indicates BioLegend citation)

Almagro S, et al. 2010. Mol. Cell Biol. 30:1703. (WB, IF, IP)
Zhang F, et al. 2004. J. Biol. Chem. 279:11760. (WB)
Iurlaro M, et al. 2004. Am. J. Pathol. 165:181. (IF)
Corada M, et al. 2001. Blood 97:1679. (IP, Block)
Kooistra M, et al. 2005. FEBS 579:4966. (Block)
Corada M, et al. 2001. Blood 97:1679. (Block)
Bouillet L, et al. 2013. Laboratory Investigation 93:1194-11202.

RRID

AB_2563669 (BioLegend Cat. No. 348513) AB_2563670 (BioLegend Cat. No. 348514)

Antigen Details

Structure: Member of the cadherin family; calcium-dependent transmembrane cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region
Distribution: Endothelial cells, hematopoietic stem cells, perineurial cells
Function: Mediates calcium-dependent homophilic cell adhesion, plays fundamental roles in microvascular permeability and in the morphogenic and proliferative events associated with angiogenesis
Ligand/Receptor: VE-cadherin
Cell Type: Endothelial cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
Antigen References: 1. Taddei A, et al. 2008. Nat. Cell Biol. 10:923.
2. Gavard J, et al. 2006. Nat. Cell Biol. 8:1223.
3. Kim I, et al. 2005. Blood 106:903.
4. Suzuki S, et al. 1991. Cell Regul. 2:261.
Gene ID: 1003 View all products for this Gene ID
UniProt: View information about CD144 on UniProt.org

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