Ultra-LEAF™ Purified anti-mouse CD144 (VE-ca-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2772	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

VE-cadherin-Ig fusion protein

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
WB, ICC, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM⁵. Additional reported applications (for relevant formats) include: Western blotting¹, blocking of cell interactions in vivo¹, and immunofluorescence microscopy^?staining⁴.

Application References (PubMed link indicates BioLegend citation)

Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block)
Liao F, et al. 2000. Cancer Res. 60:6805. (FC)
Crosby CV, et al. 2005. Blood 105:2771. (FC)
Liao F, et al. 2002. Cancer Res. 62:2567. (IF)
May C, et al. 2005. Blood 105:4337. (epitope)

RRID

AB_2888759 (BioLegend Cat. No. 138019) AB_2888759 (BioLegend Cat. No. 138020)

Antigen Details

Structure: A 120 kD member of type II Cadherin family
Distribution: Endothelial cells
Function: Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion
Ligand/Receptor: CD144
Cell Type: Endothelial cells, Mesenchymal Stem Cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821.
2. Hirashima M, et al. 2009. Blood 93:1253.
3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362.
4. Matsumura K, et al. 2003. Blood 101:1367.
5. Hirashima M, et al. 2009. Blood 101:2261.
6. Gotsch U, et al. 1997. J. Cell Sci. 110:583.
7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.
Gene ID: 12562 View all products for this Gene ID
UniProt: View information about CD144 on UniProt.org

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