Brilliant Violet 421™ anti-mouse CD144 (VE-c-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 3036	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

VE-cadherin-Ig fusion protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM⁵. Additional reported applications (for relevant formats) include: Western blotting¹, blocking of cell interactions in vivo¹, and immunofluorescence microscopy^?staining⁴.

Application References (PubMed link indicates BioLegend citation)

Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block)
Liao F, et al. 2000. Cancer Res. 60:6805. (FC)
Crosby CV, et al. 2005. Blood 105:2771. (FC)
Liao F, et al. 2002. Cancer Res. 62:2567. (IF)
May C, et al. 2005. Blood 105:4337. (epitope)

Product Citations

Krausgruber T, et al. 2020. Nature. 583:296. PubMed
Liu X, et al. 2021. Nat Commun. 12:1832. PubMed
Tang W, et al. 2021. Front Cell Dev Biol. 9:728057. PubMed
Figueiredo CA, et al. 2022. J Neuroinflammation. 19:17. PubMed

RRID

AB_2562627 (BioLegend Cat. No. 138013)

Antigen Details

Structure: A 120 kD member of type II Cadherin family
Distribution: Endothelial cells
Function: Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion
Ligand/Receptor: CD144
Cell Type: Endothelial cells, Mesenchymal Stem Cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821.
2. Hirashima M, et al. 2009. Blood 93:1253.
3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362.
4. Matsumura K, et al. 2003. Blood 101:1367.
5. Hirashima M, et al. 2009. Blood 101:2261.
6. Gotsch U, et al. 1997. J. Cell Sci. 110:583.
7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.
Gene ID: 12562 View all products for this Gene ID
UniProt: View information about CD144 on UniProt.org

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