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Purified anti-BrdU Antibody
产品名称:
Purified anti-BrdU Antibody
产品类别:
抗体
产品编号:
339801
产品应用:
339801
[价格]
规格 价格 库存
25µg ¥ 780 1

产品详情

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
IHC-P - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications include: immunohistochemical staining of formalin fixed paraffin embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Magaud J-P, et al. 1989. J. Histochem. Cytochem. 37:1517.
  2. Ejassi-Lassallette A, et al. 2011. 22:245. PubMed
  3. Ma L, et al. 2012 PNAS. 109:2090. PubMed.
Product Citations
  1. AR F, et al. 2016. Sci Rep. 6: 32900. PubMed
  2. Ejlassi-Lassallette A, et al. 2011. Mol Biol Cell. 1.086805556. PubMed
  3. Yoon J, et al. 2022. Cell Death Differ. . PubMed
  4. Ma L, et al. 2012. Proc Natl Acad Sci U S A. 109:2090. PubMed
RRID
AB_1595600 (BioLegend Cat. No. 339801) AB_1595495 (BioLegend Cat. No. 339802)

Antigen Details

Structure
Uridine derivative that can be incorporated into DNA and substitute for thymidine residues.
Distribution

Cells can be pulsed labeled with BrdU which will be incorporated into DNA during the synthesis phase of the cell cycle.

Function
Antibody against BrdU can be used to identify cells undergoing DNA replication during the period of BrdU incorporation.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Gratzner HG. 1982. Science 218:474.
2. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
3. Dolbeare F, et al. 1985. Cytometry 6:521.

Gene ID
NA
UniProt
View information about BrdU on UniProt.org

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