Purified anti-BrdU Antibody
- 产品名称:
- Purified anti-BrdU Antibody
- 产品类别:
- 抗体
- 产品编号:
- 339801
- 产品应用:
- 339801
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
IHC-P - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications include: immunohistochemical staining of formalin fixed paraffin embedded tissue sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Magaud J-P, et al. 1989. J. Histochem. Cytochem. 37:1517.
- Ejassi-Lassallette A, et al. 2011. 22:245. PubMed
- Ma L, et al. 2012 PNAS. 109:2090. PubMed.
- Product Citations
-
- AR F, et al. 2016. Sci Rep. 6: 32900. PubMed
- Ejlassi-Lassallette A, et al. 2011. Mol Biol Cell. 1.086805556. PubMed
- Yoon J, et al. 2022. Cell Death Differ. . PubMed
- Ma L, et al. 2012. Proc Natl Acad Sci U S A. 109:2090. PubMed
- RRID
- AB_1595600 (BioLegend Cat. No. 339801) AB_1595495 (BioLegend Cat. No. 339802)
- Structure
- Uridine derivative that can be incorporated into DNA and substitute for thymidine residues.
- Distribution
-
Cells can be pulsed labeled with BrdU which will be incorporated into DNA during the synthesis phase of the cell cycle.
- Function
- Antibody against BrdU can be used to identify cells undergoing DNA replication during the period of BrdU incorporation.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Immunology
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Gratzner HG. 1982. Science 218:474.
2. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
3. Dolbeare F, et al. 1985. Cytometry 6:521.
- Gene ID
- NA
- UniProt
- View information about BrdU on UniProt.org