Biotin anti-BrdU Antibody
- 产品名称：
- Biotin anti-BrdU Antibody
- 产品类别：
- 抗体
- 产品编号：
- 339809
- 产品应用：
- 339809
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications include: immunohistochemical staining of formalin fixed paraffin embedded tissue sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Magaud J-P, et al. 1989. J. Histochem. Cytochem. 37:1517.
- Tomihari M, et al. 2010. Cancer Res. 70:5778. PubMed
- Wong EB, et al. 2012. J. Immunol. 189:5667. PubMed.
- Product Citations
-
- Ghosh D, et al. 2020. J Cell Sci. . PubMed
- Anthony SM, et al. 2021. eLife. 10:00. PubMed
- Tomihari M, et al. 2010. Cancer Res. 70:5778. PubMed
- Wong E, et al. 2012. J Immunol. 189:5667. PubMed
- Ghosh D, et al. 2020. J Cell Sci. 133:00:00. PubMed
- Morita A, et al. 2018. Dev Dyn. 247:699. PubMed
- Jiang C, et al. 2021. Nat Commun. 1308:12. PubMed
- RRID
- AB_1595579 (BioLegend Cat. No. 339809) AB_1595472 (BioLegend Cat. No. 339810)
- Structure
- Uridine derivative that can be incorporated into DNA and substitute for thymidine residues.
- Distribution
-
Cells can be pulsed labeled with BrdU which will be incorporated into DNA during the synthesis phase of the cell cycle.
- Function
- Antibody against BrdU can be used to identify cells undergoing DNA replication during the period of BrdU incorporation.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Immunology
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Gratzner HG. 1982. Science 218:474.
2. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
3. Dolbeare F, et al. 1985. Cytometry 6:521.
- Gene ID
- NA
- UniProt
- View information about BrdU on UniProt.org
