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APC anti-BrdU Antibody
产品名称:
APC anti-BrdU Antibody
产品类别:
抗体
产品编号:
339808
产品应用:
339808
[价格]
规格 价格 库存
100tests ¥ 4056 1

产品详情

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications include: immunohistochemical staining of formalin fixed paraffin embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Magaud JP, et al. 1989. J. Histochem. Cytochem. 37:1517.
  2. Ejassi-Lassallette A, et al. 2011. Mol. Biol. Cell 22:245. PubMed
  3. Tan YS, et al. 2014. PLoS One. 9:111777. PubMed
Product Citations
  1. Ancey PB, et al. 2021. Cancer Res. 81:2345. PubMed
  2. Morrison K, et al. 2019. Cell Rep. 29:2961. PubMed
  3. Matsuoka S, et al. 2019. Haematologica. 105:226. PubMed
  4. Kamei A, et al. 2016. Proc Natl Acad Sci U S A. 113: E6153 - E6161. PubMed
  5. Sundaravinayagam D et al. 2019. Cell Rep. 28(6):1389-1399 . PubMed
  6. Ferrandino F, et al. 2018. Oncogene. 37:6285. PubMed
  7. Paschalis EI, et al. 2018. Proc Natl Acad Sci U S A. 115:E11359. PubMed
  8. Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed
  9. Tan Y, et al. 2014. PLoS One. 9:111777. PubMed
  10. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  11. Branigan TB, et al. 2021. Cell Reports. 34(9):108808. PubMed
  12. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  13. Xing M, et al. 2020. Molecular Cell. 78(4):714-724. PubMed
  14. Rochford I, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L686. PubMed
  15. Joshi JC, et al. 2020. Cell Rep. 30:4096. PubMed
  16. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
RRID
AB_10900446 (BioLegend Cat. No. 339807) AB_10895898 (BioLegend Cat. No. 339808)

Antigen Details

Structure
Uridine derivative that can be incorporated into DNA and substitute for thymidine residues.
Distribution
Cells can be pulsed labeled with BrdU which will be incorporated into DNA during the synthesis phase of the cell cycle.
Function
Antibody against BrdU can be used to identify cells undergoing DNA replication during the period of BrdU incorporation.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Gratzner HG. 1982. Science 218:474.
2. Dolbeare F, et al. 1983. P. Natl. Acad. Sci. USA 80:5573.
3. Dolbeare F, et al. 1985. Cytometry 6:521.

Gene ID
NA
UniProt
View information about BrdU on UniProt.org

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