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Purified anti-HDAC1 Phospho (Ser406) Antibody
产品名称:
Purified anti-HDAC1 Phospho (Ser406) Antibody
产品类别:
抗体
产品编号:
611001
产品应用:
611001
[价格]
规格 价格 库存
25µg ¥ 1344 1

产品详情

Product Details

Verified Reactivity
Human, Zebrafish
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human HDAC1 phosphorylated at Serine 406
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 ?g per ml.?For intracellular flow cytometry using our True-Phos? Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When tested for western blot, this clone produced a band that showed a ~1-3 kD mass shift compared to a pan HDAC1 antibody. This observation is consistent with a previous study of the HDAC1 Phospho (Ser406) site.

This clone recognizes zebrafish HDAC1 phosphorylated at Ser4062.

Clone?BT-15 cross-reacts to Zebrafish2

Application References

(PubMed link indicates BioLegend citation)
  1. Segre CV, et al, 2016. mAbs. 8: 37-42
  2. Loponte S, et al, 2016. Sci Rep, 6: 30213.
RRID
AB_2801111 (BioLegend Cat. No. 611001) AB_2801112 (BioLegend Cat. No. 611002)

Antigen Details

Structure
HDAC1 is a 482 amino acid protein with a predicted molecular weight of 55 kD.
Distribution

Nucleus/Ubiquitously expressed

Function
Phosphorylation of HDAC1 Ser406 results in reduced deactylase activity of the enzyme.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References
  1. Segre CV, et al, 2016. mAbs. 8: 37-42.
  2. Loponte S, et al, 2016. Sci Rep, 6: 30213.
Gene ID
3065 View all products for this Gene ID
UniProt
View information about HDAC1 Phospho Ser406 on UniProt.org

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