Purified anti-HDAC1 Phospho (Ser406) Antibody
- 产品名称:
- Purified anti-HDAC1 Phospho (Ser406) Antibody
- 产品类别:
- 抗体
- 产品编号:
- 611001
- 产品应用:
- 611001
- Verified Reactivity
- Human, Zebrafish
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human HDAC1 phosphorylated at Serine 406
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 ?g per ml.?For intracellular flow cytometry using our True-Phos? Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
When tested for western blot, this clone produced a band that showed a ~1-3 kD mass shift compared to a pan HDAC1 antibody. This observation is consistent with a previous study of the HDAC1 Phospho (Ser406) site.
This clone recognizes zebrafish HDAC1 phosphorylated at Ser4062.
Clone?BT-15 cross-reacts to Zebrafish2
- Application References
(PubMed link indicates BioLegend citation) -
- Segre CV, et al, 2016. mAbs. 8: 37-42
- Loponte S, et al, 2016. Sci Rep, 6: 30213.
- RRID
- AB_2801111 (BioLegend Cat. No. 611001) AB_2801112 (BioLegend Cat. No. 611002)
- Structure
- HDAC1 is a 482 amino acid protein with a predicted molecular weight of 55 kD.
- Distribution
-
Nucleus/Ubiquitously expressed
- Function
- Phosphorylation of HDAC1 Ser406 results in reduced deactylase activity of the enzyme.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
-
- Segre CV, et al, 2016. mAbs. 8: 37-42.
- Loponte S, et al, 2016. Sci Rep, 6: 30213.
- Gene ID
- 3065 View all products for this Gene ID
- UniProt
- View information about HDAC1 Phospho Ser406 on UniProt.org