Alexa Fluor® 488 anti-HDAC1 Phospho (Ser406) A
- 产品名称：
- Alexa Fluor® 488 anti-HDAC1 Phospho (Ser406) A
- 产品类别：
- 抗体
- 产品编号：
- 611005
- 产品应用：
- 611005
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2052 |
1 |
- Verified Reactivity
- Human, Zebrafish
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human HDAC1 phosphorylated at Serine 406
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our?True-Phos? Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
When tested for western blot, this clone produced a band that showed a ~1-3 kD mass shift compared to a pan HDAC1 antibody. This observation is consistent with a previous study of the HDAC1 Phospho (Ser406) site.
This clone recognizes zebrafish HDAC1 phosphorylated at Ser4062.
Clone?BT-15 cross-reacts to Zebrafish2
- Application References
(PubMed link indicates BioLegend citation) -
- Segre CV, et al, 2016. mAbs. 8: 37-42
- Loponte S, et al, 2016. Sci Rep, 6: 30213.
- RRID
- AB_2861046 (BioLegend Cat. No. 611005) AB_2861047 (BioLegend Cat. No. 611006)
- Structure
- HDAC1 is a 482 amino acid protein with a predicted molecular weight of 55 kD.
- Distribution
-
Nucleus/Ubiquitously expressed
- Function
- Phosphorylation of HDAC1 Ser406 results in reduced deactylase activity of the enzyme.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
-
- Segre CV, et al, 2016. mAbs. 8: 37-42.
- Loponte S, et al, 2016. Sci Rep, 6: 30213.
- Gene ID
- 3065 View all products for this Gene ID
- UniProt
- View information about HDAC1 Phospho Ser406 on UniProt.org
