TotalSeq™-B1016 anti-mouse CD157 (BST-1) Ant-上海雅吉生物科技有限公司

规格	价格	库存
10µg	￥ 4428	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Murine pre-B cell line 18.81

Purity

The antibody was purified by chromatography and conjugated with TotalSeq?-B oligomer under optimal conditions.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-B antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.

Additional Product Notes

TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNN (PCR handle), and NNNNNNNNNGCTTTAAGGCCGGTCCTAGC*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

Application References (PubMed link indicates BioLegend citation)

McNagny KM, et al. 1988. J. Immunol. 141:2551. (FC)

RRID

AB_2892293 (BioLegend Cat. No. 140211)

Antigen Details

Structure: Member of the ADP-ribosyl cyclase family, ectoenzyme, 40-46 kD, glycosyl-phosphatidylinositol (GPI)- anchored membrane protein, present on the cell surface as a homodimer
Distribution: Expressed by endothelial cells, follicular dendritic cells, cells from the myeloid lineage, and T and B cell precursors prior to the rearrangement of the antigen receptor. Expressed in many cell types from different tissues like the bone marrow, spleen, thymus, lung, kidney and heart.
Function: Synthesizes cyclic ADP-ribose (cADPR), a second messenger that elicits calcium release from intracellular stores.
Cell Type: B cells, Dendritic cells, Endothelial cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Yamamoto-Katayama S, et al. 2002. J. Mol. Biol. 310:711.
2. Funaro A, et al. 2009. Front. Biosci. 14:929.
3. Malavasi F, et al. 2006. Mol. Med. 12:334.
4. Vicari AP, et al. 1996. Int. Immunol. 8:183.
Gene ID: 12182 View all products for this Gene ID
UniProt: View information about CD157 on UniProt.org

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