APC anti-mouse CD100 (SEMA4D) Antibody
- 产品名称：
- APC anti-mouse CD100 (SEMA4D) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 147605
- 产品应用：
- 147605
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD100-Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications for the relevant formats include: immunoprecipitation from cell lysates2, capture antibody function in a sandwich ELISA assay2, and immunohistochemical staining of frozen brain sections4.
- Application References
(PubMed link indicates BioLegend citation) -
- Kumanogoh A, et al. 2000. Immunity. 13:621. (FC)
- Wang X, et al. 2001. Blood. 97:3498. (FC, IP, ELISA)
- Kumanogoh A, et al. 2002. J. Immunol. 169:1175. (FC)
- Moreau-Fauvarque C, et al. 2003 J. Neurosci. 23:9229. (IHC)
- Product Citations
-
- James OJ, et al. 2021. Nat Commun. 12:4290. PubMed
- RRID
- AB_2563615 (BioLegend Cat. No. 147605)
- Structure
- Type I transmembrane protein, one sema domain, one C2-type immunoglobulin domain, homodimer, 150 kD.
- Distribution
-
Highly expressed on most hematopoietic cells, is also found in the brain, kidney, and heart. Overexpressed by some malignant cell types.
- Function
- Potent inducer of angiogenesis, inhibitor of bone formation, plays a role in lymphocyte activation, antibody production and dendritic cell maturation. In neurons, regulates dendrite branching and axon guidance.
- Ligand/Receptor
- CD72, Plexin-B1.
- Cell Type
- B cells, Dendritic cells, Neutrophils, Tregs
- Biology Area
- Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Signal Transduction, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell. 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
- Gene ID
- 20354 View all products for this Gene ID
- UniProt
- View information about CD100 on UniProt.org
