Purified anti-mouse F4/80 (Maxpar® Ready) Anti-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1548	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Murine macrophages

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^1,2 and formalin-fixed paraffin-embedded sections^6,7, Western blotting, and spatial biology (IBEX)^12,13.

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

McDonald B, et al. 2020. Cell Host Microbe. 28(5):660-668.e4. PubMed
Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed

RRID

AB_2563767 (BioLegend Cat. No. 123143)

Antigen Details

Structure: EGF-TM7 family member, 160 kD glycoprotein
Distribution: Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells
Function: Induction of immunological tolerance
Cell Type: Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References: 1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.
Gene ID: 13733 View all products for this Gene ID
UniProt: View information about F4/80 on UniProt.org

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