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首页 > 抗体蛋白 > 抗体
PerCP anti-mouse F4/80 Antibody
产品名称：
PerCP anti-mouse F4/80 Antibody
产品类别：
抗体
产品编号：
123125
产品应用：
123125
[价格]
规格 价格 库存
25µg ￥ 1548 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  13. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Kiepura A, et al. 2021. Int J Mol Sci. 22:. PubMed
  2. DeSouza-Vieira T, et al. 2020. Cell Rep. 33:108317. PubMed
  3. Lee S, et al. 2019. eLife. 0.333333333333333. PubMed
  4. Lyons J, et al. 2016. PLoS One. 11: 0149069. PubMed
  5. Antunes KH, et al. 2022. Front Immunol. 13:867022. PubMed
  6. Forni MF, et al. 2021. Front Oncol. 11:667715. PubMed
  7. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  8. Ho T, et al. 2011. J Biol Chem. 286:35943. PubMed
  9. Fan X, et al. 2015. Mol Cancer Ther. 14:681. PubMed
  10. Zhang S, et al. 2012. Infect Immun . 80:3952. PubMed
  11. Wang ECE, et al. 2019. Cell Stem Cell. 24:654. PubMed
  12. Burkhardt A, et al. 2014. J Immunol. 193:1468. PubMed
  13. Aguilera M, et al. 2021. Int J Mol Sci. 22:. PubMed
  14. Olivo Pimentel V, et al. 2021. J Immunother Cancer. 9:. PubMed
  15. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  16. Soukup K, et al. 2017. Sci Rep. 10.1038/s41598-017-12208-7. PubMed
  17. Laurent J, et al. 2011. Cancer Res. 71:3781. PubMed
  18. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  19. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
RRID
AB_893495 (BioLegend Cat. No. 123125) AB_893483 (BioLegend Cat. No. 123126)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

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