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PE/Cyanine5 anti-mouse F4/80 Antibody
产品名称:
PE/Cyanine5 anti-mouse F4/80 Antibody
产品类别:
抗体
产品编号:
123112
产品应用:
123112
[价格]
规格 价格 库存
100µg ¥ 3516 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  13. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  2. Shakya S, et al. 2020. MethodsX. 7:101027. PubMed
  3. R?dahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  4. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  5. Han H, et al. 2021. Front Pharmacol. 12:726586. PubMed
  6. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  7. Hays C, et al. 2020. eLife. 8:e48772.. PubMed
  8. Yang YL, et al. 2019. J Virol. 93:e01448-19. PubMed
  9. Hering L, et al. 2021. Int J Mol Sci. 22:. PubMed
  10. Kan C, et al. 2022. Bone Res. 10:62. PubMed
  11. Yang R, et al. 2012. J Immunol. 189:2656. PubMed
  12. Zhang J, et al. 2022. iScience. 25:104963. PubMed
  13. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  14. Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed
  15. Garcia LR, et al. 2021. Nat Commun. 12:3364. PubMed
  16. Johnson LA, et al. 2021. Life Sci Alliance. :4. PubMed
  17. Xiao Y, et al. 2018. Int Immunol. 30:457. PubMed
  18. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  19. Desai P, et al. 2021. Cell. 184(5):1214-1231.e16. PubMed
  20. Zuo Y, et al. 2020. Small GTPases. 0.661805556. PubMed
  21. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  22. Shen L, et al. 2016. Sci Rep. 6:30347. PubMed
  23. Lai SM et al. 2018. Cell reports. 25(11):3099-3109 . PubMed
  24. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  25. Utz SG, et al. 2020. Cell. 181(3):557-573. PubMed
  26. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  27. RL M, et al. 2015. Proc Natl Acad Sci U S A. 112:6506-6514. PubMed
  28. Shen L, et al. 2021. Cell Metabolism. 33(5):971-987.e6. PubMed
RRID
AB_893494 (BioLegend Cat. No. 123111) AB_893482 (BioLegend Cat. No. 123112)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

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