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Brilliant Violet 650™ anti-mouse F4/80 Antib
产品名称：
Brilliant Violet 650™ anti-mouse F4/80 Antib
产品类别：
抗体
产品编号：
123149
产品应用：
123149
[价格]
规格 价格 库存
50µg ￥ 3300 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650? under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650? excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  13. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
  2. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  3. Maisonneuve C, et al. 2021. Cell Reports. 34(4):108677. PubMed
  4. Godbersen-Palmer C, et al. 2020. J Immunol. 204:2973. PubMed
  5. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  6. Francian A, et al. 2021. J Drug Target. 29:754. PubMed
  7. Wang C, et al. 2021. Cell Stem Cell. . PubMed
  8. Law HL, et al. 2020. Front Pharmacol. 11:274. PubMed
  9. Fulham MA, et al. 2019. Am J Physiol Cell Physiol. 317:C687. PubMed
  10. Hegde S, et al. 2020. Cancer Cell. 37(3):289-307. PubMed
  11. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  12. Rhys HI, et al. 2018. EBioMedicine. 29:60. PubMed
  13. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  14. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  15. Campesato LF, et al. 2020. Nat Commun. 3.24375. PubMed
  16. Tang S, et al. 2022. Virol J. 19:32. PubMed
  17. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  18. Chen KE, et al. 2021. J Immunol. 206:141. PubMed
  19. Chen KE, et al. 2021. J Neuroinflammation. 18:140. PubMed
  20. Zhang L, et al. 2020. Cell. 442:181. PubMed
  21. Budhu S, et al. 2021. Cell Reports. 34(2):108620. PubMed
  22. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
  23. Samuelson DR, et al. 2021. Commun Biol. 4:997. PubMed
  24. Davis FM, et al. 2021. J Exp Med. 218:. PubMed
RRID
AB_2564589 (BioLegend Cat. No. 123149)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

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