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首页 > 抗体蛋白 > 抗体
Biotin anti-mouse F4/80 Antibody
产品名称：
Biotin anti-mouse F4/80 Antibody
产品类别：
抗体
产品编号：
123105
产品应用：
123105
[价格]
规格 价格 库存
50µg ￥ 708 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  13. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  2. Sasaki O, et al. 2013. J Immunol. 191:2879. PubMed
  3. Knab L, et al. 2014. Mol Cancer Res. 12:1440. PubMed
  4. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  5. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  6. Boccasavia VL, et al. 2021. Cell Reports. 34(11):108861. PubMed
  7. Krueger PD, et al. 2021. Immunity. 54(4):687-701.e4. PubMed
  8. Yuan D et al. 2017. Cancer cell. 31(6):771-789 . PubMed
  9. Iturri L, et al. 2021. Immunity. . PubMed
  10. Hazama D, et al. 2020. Cell Chemical Biology. 27(9):1181-1191.e7. PubMed
  11. Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
  12. Georgilis A et al. 2018. Cancer cell. 34(1):85-102 . PubMed
  13. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  14. Xu S, et al. 2016. PLoS One. 11: 0163829. PubMed
  15. Wu W, et al. 2016. PLoS One. 11: 0159512. PubMed
  16. Goggi JL, et al. 2020. Mol Imaging Biol. 22:1392. PubMed
  17. Thion MS et al. 2018. Cell. 172(3):500-516 . PubMed
  18. Yauch L, et al. 2010. J Immunol. 185:5405. PubMed
  19. Zoller E, et al. 2011. J Exp Med. 208:1203. PubMed
  20. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  21. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  22. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  23. Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
  24. Naing A et al. 2019. Cell reports. 26(5):1242-1257 . PubMed
  25. Linde N, et al. 2018. Nat Commun. 9:21. PubMed
  26. Kim AD, et al. 2021. Sci Rep. 11:24194. PubMed
  27. Onodera T, et al. 2021. Immunity. 54:2385. PubMed
  28. Zeng Z, et al. 2022. Oncogene. :. PubMed
  29. Akk A, et al. 2019. Mol Immunol. 114:629. PubMed
  30. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  31. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  32. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  33. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  34. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  35. Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
  36. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  37. Grayczyk JP et al. 2017. Cell host & microbe. 22(5):678-687 . PubMed
  38. Werner A, et al. 2021. iScience. 24:103076. PubMed
  39. Matsumoto S, et al. 2010. J Immunol. 184:1543. PubMed
  40. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  41. Lutz J, et al. 2015. Nat Commun. 6: 8575. PubMed
  42. Calvente CJ, et al. 2019. J Clin Invest. 130:4091. PubMed
  43. Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed
  44. Liao YC, et al. 2021. Front Immunol. 12:743030. PubMed
  45. Moriyasu T, et al. 2018. PLoS Negl Trop Dis. 12:e0006197. PubMed
RRID
AB_893499 (BioLegend Cat. No. 123105) AB_893501 (BioLegend Cat. No. 123106)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

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