APC/Fire™ 750 anti-mouse F4/80 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1836	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Murine macrophages

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire? 750 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire? 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^1,2 and formalin-fixed paraffin-embedded sections^6,7, Western blotting, and spatial biology (IBEX)^12,13.

Application References (PubMed link indicates BioLegend citation)

Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Marangoni F, et al. 2021. Cell. . PubMed
Shane HL, et al. 2020. Food Chem Toxicol. 136:111114. PubMed
Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
Danzer H, et al. 2020. Elife. 9:00. PubMed
Chakraborty A, et al. 2021. Biomaterials. 273:120796. PubMed

RRID

AB_2616724 (BioLegend Cat. No. 123151) AB_2616725 (BioLegend Cat. No. 123152)

Antigen Details

Structure: EGF-TM7 family member, 160 kD glycoprotein
Distribution: Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells
Function: Induction of immunological tolerance
Cell Type: Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References: 1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.
Gene ID: 13733 View all products for this Gene ID
UniProt: View information about F4/80 on UniProt.org

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