热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
Alexa Fluor® 700 anti-mouse F4/80 Antibody
产品名称：
Alexa Fluor® 700 anti-mouse F4/80 Antibody
产品类别：
抗体
产品编号：
123129
产品应用：
123129
[价格]
规格 价格 库存
25µg ￥ 1716 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  13. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  2. D?britz J, et al. 2016. Sci Rep. 6:20584. PubMed
  3. Domingues CS, et al. 2020. Front Immunol. 11:566476. PubMed
  4. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  5. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  6. Lindborg JA et al. 2017. The Journal of Neuroscience. 37(43):10258-10277 . PubMed
  7. Tuit S, et al. 2019. Cell Rep. 29:1221. PubMed
  8. Dumas AA, et al. 2020. EMBO J. 39:e103790. PubMed
  9. Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
  10. Casadesús AV, et al. 2020. Oncoimmunology. 9:1770565. PubMed
  11. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  12. Redelinghuys P, et al. 2016. Ann Rheum Dis. 75: 1386 - 1391. PubMed
  13. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  14. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  15. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  16. Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed
  17. Buzzelli JN, et al. 2019. Am J Physiol Gastrointest Liver Physiol. 316:G251. PubMed
  18. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  19. Ghorbani S, et al. 2022. Nat Commun. 13:2445. PubMed
  20. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  21. Dholakia J, et al. 2022. Gynecol Oncol. 164:170. PubMed
  22. Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
  23. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
  24. Liu Y, et al. 2019. Sci Rep. 9:18970. PubMed
  25. Barsoumian HB, et al. 2022. Cancers (Basel). 14:. PubMed
  26. Smith LK, et al. 2021. Elife. 10:. PubMed
  27. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  28. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  29. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
RRID
AB_2277848 (BioLegend Cat. No. 123129) AB_2293450 (BioLegend Cat. No. 123130)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线